EVCOUNT vs NTA
Why advanced flow cytometry is the superior choice for complex clinical samples.
Going Beyond Simple Particle Counting
While Nanoparticle Tracking Analysis (NTA) is a common tool for bulk particle sizing, it faces significant limitations when analyzing complex biological fluids like blood plasma. At EVcount, our advanced flow cytometry platform overcomes these barriers, providing not just physical measurements, but deep biological insights at the single-vesicle level.
NTA
EVCOUNT
Measures all light-scattering particles in a sample. In body fluids, EVs are vastly outnumbered by lipoproteins and protein aggregates, making it difficult for NTA to distinguish true EVs from background noise.
Utilizes multi-label fluorescence to specifically identify EVs. Built-in assay controls ensure that signals truly originate from EVs, eliminating background interference.
Primarily provides bulk data on particle size distribution and total concentration.
Simultaneously delivers precise measurements of EV size, concentration, cellular origin, and functional markers (e.g., markers of activation or cell death) for each individual vesicle.
Lack of standardized calibration across different instruments often leads to variability in measurements, hampering clinical validation.
Operates on an ISO 15189-certified workflow with robust metrological calibration, delivering highly reproducible, MIFlowCyt-EV-compliant data ready for publication and clinical research.
EVcount is currently operating under the umbrella of Amsterdam University Medical Center to assess business feasibility. The activities are conducted under the supervision of the technology transfer office.
